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MEK1 Monoclonal antibody

MEK1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3F6D7

Cat no : 67872-1-Ig

Synonyms

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1, PRKMK1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of A431 using 67872-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (MEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of HeLa using 67872-1-Ig

    Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of HeLa using 67872-1-Ig

    WB result of MEK1 antibody (67872-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEK1 transfected HeLa cells.

  • Western Blot (WB) analysis of PC-3 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of PC-3 using 67872-1-Ig

    Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of NIH/3T3 using 67872-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using MEK1 Monoclonal antibody (67872-1-Ig)

    WB analysis of HSC-T6 using 67872-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, Calyculin A treated HeLa cells, NIH/3T3 cells, HSC-T6 cells, Calyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67872-1-Ig targets MEK1 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC139729
Gene Symbol MEK1
Gene ID (NCBI) 5604
RRIDAB_2918630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.

Protocols

Product Specific Protocols
WB protocol for MEK1 antibody 67872-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Ethnopharmacol

Jia-Wei-Si-Miao-Yong-An Fang stimulates the healing of acute radiation-induced cutaneous wounds through MAPK/ERK pathway

Authors - Yin Wang
View Article