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HABP2 Monoclonal antibody

HABP2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A7C2

Cat no : 67867-1-Ig

Synonyms

FSAP, Factor VII-activating protease, Factor VII activating protease, Factor seven-activating protease, EC:3.4.21.-

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using HABP2 Monoclonal antibody (67867-1-Ig)

    WB analysis of human plasma using 67867-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 67867-1-Ig (HABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HABP2 Monoclonal antibody (67867-1-Ig)

    IF Staining of HepG2 using 67867-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HABP2 antibody (67867-1-Ig, Clone: 1A7C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using HABP2 Monoclonal antibody (67867-1-Ig)

    FC experiment of HepG2 using 67867-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug HABP2 Monoclonal antibody (67867-1-Ig, Clone:1A7C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman plasma
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67867-1-Ig targets HABP2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HABP2 fusion protein Ag3981
Full Name hyaluronan binding protein 2
Calculated Molecular Weight 560 aa, 63 kDa
Observed Molecular Weight60,63 kDa
GenBank Accession NumberBC031412
Gene Symbol HABP2
Gene ID (NCBI) 3026
RRIDAB_2918625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HABP2 antibody 67867-1-IgDownload protocol
IF protocol for HABP2 antibody 67867-1-IgDownload protocol
FC protocol for HABP2 antibody 67867-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols