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GSTM1 Monoclonal antibody

GSTM1 Monoclonal Antibody for WB, ELISA, FC (Inrta)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, chicken, mouse, rat, pig

Applications

WB, ELISA, FC (Inrta)

Conjugate

Unconjugated

CloneNo.

3C5C2

Cat no : 67862-1-Ig

Synonyms

GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1, EC:2.5.1.18

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of rat liver using 67862-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of mouse liver using 67862-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of chicken liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of chicken liver using 67862-1-Ig

    chicken liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of HeLa using 67862-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of Jurkat using 67862-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of pig liver using 67862-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using GSTM1 Monoclonal antibody (67862-1-Ig)

    FC experiment of HeLa using 67862-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GSTM1 (67862-1-Ig, Clone:3C5C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inrat liver tissue, HeLa cells, Jurkat cells, pig liver tissue, mouse liver tissue, chicken liver tissue
Positive FC (Inrta) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INRTA)FC (INRTA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67862-1-Ig targets GSTM1 in WB, ELISA, FC (Inrta) applications and shows reactivity with Human, chicken, mouse, rat, pig samples.

Tested Reactivity Human, chicken, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GSTM1 fusion protein Ag3113
Full Name glutathione S-transferase mu 1
Calculated Molecular Weight 181 aa, 21 kDa
Observed Molecular Weight26-28 kDa
GenBank Accession NumberBC024005
Gene Symbol GSTM1
Gene ID (NCBI) 2944
RRIDAB_2918620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSTM1 antibody 67862-1-IgDownload protocol
FC protocol for GSTM1 antibody 67862-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols