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  • Western Blot (WB) analysis of various lysates using GIT2 Monoclonal antibody (67858-1-Ig)

    WB analysis using 67858-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of K-562 cells using GIT2 Monoclonal antibody (67858-1-Ig)

    WB analysis of K-562 using 67858-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using GIT2 Monoclonal antibody (67858-1-Ig)

    WB analysis of HSC-T6 using 67858-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67858-1-Ig (GIT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GIT2 Monoclonal antibody (67858-1-Ig)

    IHC staining of human prostate cancer using 67858-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67858-1-Ig (GIT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GIT2 Monoclonal antibody

GIT2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67858-1-Ig
Clone No.2D9C5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Synonyms

ARF GAP GIT2, CAT 2, CAT2, DKFZp686G01261, GIT2, GRK interacting protein 2, KIAA0148

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inA549 cells, K-562 cells, HSC-T6 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67858-1-Ig targets GIT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GIT2 fusion protein Ag0321 Predict reactive species
Full Name G protein-coupled receptor kinase interacting ArfGAP 2
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC001379
Gene Symbol GIT2
Gene ID (NCBI) 9815
RRIDAB_2918616
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GIT2 antibody 67858-1-IgDownload protocol
IHC protocol for GIT2 antibody 67858-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols