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EFTUD2 Monoclonal antibody

EFTUD2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F4G2

Cat no : 67855-1-Ig

Synonyms

DKFZp686E24196, EFTUD2, FLJ44695, hSNU114, KIAA0031, Snrp116, Snu114, SNU114 homolog, U5 116 kDa, U5 116KD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EFTUD2 Monoclonal antibody (67855-1-Ig)

    WB analysis using 67855-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67855-1-Ig (EFTUD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of HeLa cells using EFTUD2 Monoclonal antibody (67855-1-Ig)

    WB analysis of HeLa using 67855-1-Ig

    WB result of EFTUD2 antibody (67855-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFTUD2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EFTUD2 Monoclonal antibody (67855-1-Ig)

    IHC staining of human liver cancer using 67855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67855-1-Ig (EFTUD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EFTUD2 Monoclonal antibody (67855-1-Ig)

    IHC staining of human liver cancer using 67855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67855-1-Ig (EFTUD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EFTUD2 Monoclonal antibody (67855-1-Ig)

    IHC staining of human colon cancer using 67855-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67855-1-Ig (EFTUD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EFTUD2 Monoclonal antibody (67855-1-Ig)

    IF Staining of HeLa using 67855-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EFTUD2 antibody (67855-1-Ig, Clone: 2F4G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67855-1-Ig targets EFTUD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EFTUD2 fusion protein Ag24345
Full Name elongation factor Tu GTP binding domain containing 2
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 109-120 kDa
GenBank Accession NumberBC002360
Gene Symbol EFTUD2
Gene ID (NCBI) 9343
RRIDAB_2918613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EFTUD2 antibody 67855-1-IgDownload protocol
IHC protocol for EFTUD2 antibody 67855-1-IgDownload protocol
IF protocol for EFTUD2 antibody 67855-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

The feedback loop of EFTUD2/c-MYC impedes chemotherapeutic efficacy by enhancing EFTUD2 transcription and stabilizing c-MYC protein in colorectal cancer

Authors - Xiaojian Zhu
View Article