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MED4 Monoclonal antibody

MED4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6F4

Cat no : 67839-1-Ig

Synonyms

ARC36, DRIP36, FLJ10956, HSPC126, MED4, mediator complex subunit 4, RP11 90M2.2, TRAP36, VDRIP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MED4 Monoclonal antibody (67839-1-Ig)

    WB analysis using 67839-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67839-1-Ig (MED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MED4 Monoclonal antibody (67839-1-Ig)

    IHC staining of human breast cancer using 67839-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67839-1-Ig (MED4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MED4 Monoclonal antibody (67839-1-Ig)

    IF Staining of HepG2 using 67839-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MED4 antibody (67839-1-Ig, Clone: 2E6F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (Blue).

Tested Applications

Positive WB detected inLNCaP cells, Hela, HEK-293, Jurkat, K-562, HSC-T6, PC-12, NIH/3T3, 4T1
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67839-1-Ig targets MED4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Reactivity Human, Mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MED4 fusion protein Ag8562
Full Name mediator complex subunit 4
Calculated Molecular Weight 270 aa, 30 kDa
Observed Molecular Weight~36 kDa
GenBank Accession NumberBC005189
Gene Symbol MED4
Gene ID (NCBI) 29079
RRIDAB_2918601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".

Protocols

Product Specific Protocols
WB protocol for MED4 antibody 67839-1-IgDownload protocol
IHC protocol for MED4 antibody 67839-1-IgDownload protocol
IF protocol for MED4 antibody 67839-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols