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RAB1B Monoclonal antibody

RAB1B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C2G6

Cat no : 67837-1-Ig

Synonyms

RAB1B, Ras related protein Rab 1B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB1B Monoclonal antibody (67837-1-Ig)

    WB analysis using 67837-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAB1B Monoclonal antibody (67837-1-Ig)

    WB analysis of NIH/3T3 using 67837-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAB1B Monoclonal antibody (67837-1-Ig)

    WB analysis of HeLa using 67837-1-Ig

    WB result of RAB1B antibody (67837-1-Ig; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RAB1B Monoclonal antibody (67837-1-Ig)

    IF Staining of MCF-7 using 67837-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAB1B Monoclonal antibody (67837-1-Ig)

    IF Staining of NIH/3T3 using 67837-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inNIH/3T3 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67837-1-Ig targets RAB1B in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAB1B fusion protein Ag12069
Full Name RAB1B, member RAS oncogene family
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC071169
Gene Symbol RAB1B
Gene ID (NCBI) 81876
RRIDAB_2918599
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB1B antibody 67837-1-IgDownload protocol
IF protocol for RAB1B antibody 67837-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols