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  • Featured Product
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PKMYT1 Monoclonal antibody

PKMYT1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D12D3

Cat no : 67806-1-Ig

Synonyms

DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKMYT1 Monoclonal antibody (67806-1-Ig)

    WB analysis using 67806-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67806-1-Ig (PKMYT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of MCF-7 cells using PKMYT1 Monoclonal antibody (67806-1-Ig)

    WB analysis of MCF-7 using 67806-1-Ig

    WB result of PKMYT1 antibody (67806-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PKMYT1 Monoclonal antibody (67806-1-Ig)

    IHC staining of human colon cancer using 67806-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67806-1-Ig (PKMYT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PKMYT1 Monoclonal antibody (67806-1-Ig)

    IHC staining of human colon cancer using 67806-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67806-1-Ig (PKMYT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67806-1-Ig targets PKMYT1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PKMYT1 fusion protein Ag30849
Full Name protein kinase, membrane associated tyrosine/threonine 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberNM_004203
Gene Symbol PKMYT1
Gene ID (NCBI) 9088
RRIDAB_2918569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)

Protocols

Product Specific Protocols
WB protocol for PKMYT1 antibody 67806-1-IgDownload protocol
IHC protocol for PKMYT1 antibody 67806-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Cell Oncol (Dordr)

ELF3 promotes gemcitabine resistance through PKMYT1/CDK1 signaling pathway in gallbladder cancer

Authors - Linhua Yang
View Article
mouseWB

Adv Sci (Weinh)

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

Authors - Kun-Huan Zhang
View Article