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TWF1 Monoclonal antibody

TWF1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F10F11

Cat no : 67796-1-Ig

Synonyms

A6, Protein A6, Protein tyrosine kinase 9, PTK9, TWF1, Twinfilin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TWF1 Monoclonal antibody (67796-1-Ig)

    WB analysis using 67796-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of Jurkat cells using TWF1 Monoclonal antibody (67796-1-Ig)

    WB analysis of Jurkat using 67796-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TWF1 Monoclonal antibody (67796-1-Ig)

    IF Staining of MCF-7 using 67796-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TWF1 antibody (67796-1-Ig, Clone: 2F10F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67796-1-Ig targets TWF1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TWF1 fusion protein Ag28630
Full Name twinfilin, actin-binding protein, homolog 1 (Drosophila)
Calculated Molecular Weight 368 aa, 42 kDa
Observed Molecular Weight36-38 kDa
GenBank Accession NumberBC022344
Gene Symbol TWF1
Gene ID (NCBI) 5756
RRIDAB_2918560
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Twinfilin-1 (TWF1) is an actin-binding protein implicated in cell motility, invasion and migration. Its expression is linked to sensitivity to drugs and cancer progression. Overexpression of TWF1 has been reported in various cancers, including breast cancer and lung cancer.

Protocols

Product Specific Protocols
WB protocol for TWF1 antibody 67796-1-IgDownload protocol
IF protocol for TWF1 antibody 67796-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols