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ABHD5 Monoclonal antibody

ABHD5 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12C2

Cat no : 67779-1-Ig

Synonyms

ABHD5, CDS, CGI58, IECN2, NCIE2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABHD5 Monoclonal antibody (67779-1-Ig)

    WB analysis using 67779-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67779-1-Ig (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ABHD5 Monoclonal antibody (67779-1-Ig)

    IF Staining of HepG2 using 67779-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ABHD5 antibody (67779-1-Ig, Clone: 2B12C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, THP-1 cells, Raji cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67779-1-Ig targets ABHD5 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ABHD5 fusion protein Ag30256
Full Name abhydrolase domain containing 5
Calculated Molecular Weight 349 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC021958
Gene Symbol ABHD5
Gene ID (NCBI) 51099
RRIDAB_2918543
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

Protocols

Product Specific Protocols
WB protocol for ABHD5 antibody 67779-1-IgDownload protocol
IF protocol for ABHD5 antibody 67779-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols