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USP25 Monoclonal antibody

USP25 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6A2

Cat no : 67769-1-Ig

Synonyms

Deubiquitinating enzyme 25, Ubiquitin thiolesterase 25, USP on chromosome 21, USP21, USP25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP25 Monoclonal antibody (67769-1-Ig)

    WB analysis using 67769-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using USP25 Monoclonal antibody (67769-1-Ig)

    WB analysis using 67769-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67769-1-Ig (USP25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using USP25 Monoclonal antibody (67769-1-Ig)

    IHC staining of mouse skeletal muscle using 67769-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using USP25 Monoclonal antibody (67769-1-Ig)

    IHC staining of mouse skeletal muscle using 67769-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67769-1-Ig (USP25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, A549 cells, HeLa cells, HepG2 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67769-1-Ig targets USP25 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen USP25 fusion protein Ag28067
Full Name ubiquitin specific peptidase 25
Calculated Molecular Weight 125 kDa
Observed Molecular Weight125 kDa
GenBank Accession NumberBC015930
Gene Symbol USP25
Gene ID (NCBI) 29761
RRIDAB_2918535
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

Protocols

Product Specific Protocols
WB protocol for USP25 antibody 67769-1-IgDownload protocol
IHC protocol for USP25 antibody 67769-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols