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PTPN14 Monoclonal antibody

PTPN14 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8C8

Cat no : 67744-1-Ig

Synonyms

PEZ, PTP36, PTPN14

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PTPN14 Monoclonal antibody (67744-1-Ig)

    WB analysis using 67744-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67744-1-Ig (PTPN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PTPN14 Monoclonal antibody (67744-1-Ig)

    IHC staining of human placenta using 67744-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PTPN14 Monoclonal antibody (67744-1-Ig)

    IHC staining of human placenta using 67744-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inU2OS cells, A549 cells, HEK-293 cells, Hela cells, K-562 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67744-1-Ig targets PTPN14 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTPN14 fusion protein Ag12679
Full Name protein tyrosine phosphatase, non-receptor type 14
Calculated Molecular Weight 1187 aa, 135 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC101754
Gene Symbol PTPN14
Gene ID (NCBI) 5784
RRIDAB_2918513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

Protocols

Product Specific Protocols
WB protocol for PTPN14 antibody 67744-1-IgDownload protocol
IHC protocol for PTPN14 antibody 67744-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Biomed Pharmacother

XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma

Authors - Zengbin Wang
View Article