PTGES3 Monoclonal antibody
PTGES3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, pig, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3C11D11
Cat no : 67736-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, PC-3 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
67736-1-Ig targets PTGES3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
| Tested Reactivity | Human, mouse, pig, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PTGES3 fusion protein Ag7870 |
| Full Name | prostaglandin E synthase 3 (cytosolic) |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC003005 |
| Gene Symbol | PTGES3 |
| Gene ID (NCBI) | 10728 |
| RRID | AB_2918505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTGES3 antibody 67736-1-Ig | Download protocol |
| IHC protocol for PTGES3 antibody 67736-1-Ig | Download protocol |
| IF protocol for PTGES3 antibody 67736-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol High expression of PTGES3 is an independent predictive poor prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma. | ||
Cell Death Discov METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition | ||
J Transl Med The integration of multidisciplinary approaches revealed PTGES3 as a novel drug target for breast cancer treatment | ||
Reprod Sci Progressively Diminished Prostaglandin E2 Signaling in Concordance with Increasing Fibrosis in Ectopic Endometrium |
