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ZNF217 Monoclonal antibody

ZNF217 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F11B6

Cat no : 67730-1-Ig

Synonyms

ZABC1, zinc finger protein 217, ZNF217

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis using 67730-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis of HEK-293 using 67730-1-Ig

    WB result of ZNF217 antibody (67730-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis using 67730-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using ZNF217 Monoclonal antibody (67730-1-Ig)

    WB analysis of 4T1 using 67730-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using ZNF217 Monoclonal antibody (67730-1-Ig)

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using ZNF217 Monoclonal antibody (67730-1-Ig)

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells, 4T1 cells, HEK-293 cells, HepG2 cells, PC-3 cells, K-562 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67730-1-Ig targets ZNF217 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ZNF217 fusion protein Ag19468
Full Name zinc finger protein 217
Calculated Molecular Weight 1048 aa, 115 kDa
Observed Molecular Weight110-130 kDa
GenBank Accession NumberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
RRIDAB_2882916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF217 antibody 67730-1-IgDownload protocol
IHC protocol for ZNF217 antibody 67730-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols