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EIF3L Monoclonal antibody

EIF3L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B11A10

Cat no : 67719-1-Ig

Synonyms

Eukaryotic translation initiation factor 3 subunit E-interacting protein, Eukaryotic translation initiation factor 3 subunit 6-interacting protein, EIF3S6IP, EIF3S11, EIF3EIP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF3L Monoclonal antibody (67719-1-Ig)

    WB analysis using 67719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67719-1-Ig (EIF3L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EIF3L Monoclonal antibody (67719-1-Ig)

    WB analysis using 67719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67719-1-Ig (EIF3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IHC staining of mouse pancreas using 67719-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IHC staining of mouse pancreas using 67719-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IF Staining of mouse pancreas using 67719-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IF Staining of mouse pancreas using 67719-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF3L Monoclonal antibody (67719-1-Ig)

    IF Staining of HeLa using 67719-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using EIF3L Monoclonal antibody (67719-1-Ig)

    FC experiment of HeLa using 67719-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EIF3L (67719-1-Ig, Clone:1B11A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells, PC-12 cells, 4T1 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67719-1-Ig targets EIF3L in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EIF3L fusion protein Ag26680
Full Name eukaryotic translation initiation factor 3, subunit L
Calculated Molecular Weight 67 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC007510
Gene Symbol EIF3L
Gene ID (NCBI) 51386
RRIDAB_2882907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF3L antibody 67719-1-IgDownload protocol
IHC protocol for EIF3L antibody 67719-1-IgDownload protocol
IF protocol for EIF3L antibody 67719-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols