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CEP290 Monoclonal antibody

CEP290 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C10G2

Cat no : 67716-1-Ig

Synonyms

3H11Ag, BBS14, Cancer/testis antigen 87, centrosomal protein 290kDa, Centrosomal protein of 290 kDa, CEP290, CT87, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, Nephrocystin 6, rd16, SLSN6, Tumor antigen se2 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP290 Monoclonal antibody (67716-1-Ig)

    WB analysis using 67716-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67716-1-Ig (CEP290 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using CEP290 Monoclonal antibody (67716-1-Ig)

    WB analysis of HSC-T6 using 67716-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67716-1-Ig (CEP290 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HSC-T6 cells, HeLa cells, NCCIT cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67716-1-Ig targets CEP290 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CEP290 fusion protein Ag17457
Full Name centrosomal protein 290kDa
Calculated Molecular Weight 2479 aa, 290 kDa
Observed Molecular Weight290 kDa
GenBank Accession NumberBC008641
Gene Symbol CEP290
Gene ID (NCBI) 80184
RRIDAB_2918499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt.

Protocols

Product Specific Protocols
WB protocol for CEP290 antibody 67716-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols