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PSMD5 Monoclonal antibody

PSMD5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, pig, rat, rabbit, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5F7

Cat no : 67682-1-Ig

Synonyms

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using PSMD5 Monoclonal antibody (67682-1-Ig)

    WB analysis of pig liver using 67682-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PSMD5 Monoclonal antibody (67682-1-Ig)

    WB analysis of K-562 using 67682-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit liver tissue using PSMD5 Monoclonal antibody (67682-1-Ig)

    WB analysis of rabbit liver using 67682-1-Ig

    rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using PSMD5 Monoclonal antibody (67682-1-Ig)

    WB analysis of rat liver using 67682-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HCT 116 cells using PSMD5 Monoclonal antibody (67682-1-Ig)

    WB analysis of HCT 116 using 67682-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMD5 Monoclonal antibody (67682-1-Ig)

    IHC staining of human colon cancer using 67682-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMD5 Monoclonal antibody (67682-1-Ig)

    IHC staining of human colon cancer using 67682-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PSMD5 Monoclonal antibody (67682-1-Ig)

    IF Staining of A431 using 67682-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inpig liver tissue, rabbit liver tissue, rat liver tissue, K-562 cells, HCT 116 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67682-1-Ig targets PSMD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

Tested Reactivity Human, pig, rat, rabbit, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen PSMD5 fusion protein Ag19298
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC014478
Gene Symbol PSMD5
Gene ID (NCBI) 5711
RRIDAB_2882875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSMD5 antibody 67682-1-IgDownload protocol
IHC protocol for PSMD5 antibody 67682-1-IgDownload protocol
IF protocol for PSMD5 antibody 67682-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols