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MAT2B Monoclonal antibody

MAT2B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7G8

Cat no : 67643-1-Ig

Synonyms

MAT II, MAT II beta, MAT2B, MATIIbeta, Nbla02999, SDR23E1, TGR, UNQ2435/PRO4995

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAT2B Monoclonal antibody (67643-1-Ig)

    WB analysis using 67643-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67643-1-Ig (MAT2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using MAT2B Monoclonal antibody (67643-1-Ig)

    WB analysis of NIH/3T3 using 67643-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67643-1-Ig (MAT2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAT2B Monoclonal antibody (67643-1-Ig)

    WB analysis using 67643-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67643-1-Ig (MAT2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MAT2B Monoclonal antibody (67643-1-Ig)

    IHC staining of human colon cancer using 67643-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67643-1-Ig (MAT2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MAT2B Monoclonal antibody (67643-1-Ig)

    IHC staining of human colon cancer using 67643-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67643-1-Ig (MAT2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells, HSC-T6 cells, LNcaP cells, U2OS cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67643-1-Ig targets MAT2B in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAT2B fusion protein Ag8649
Full Name methionine adenosyltransferase II, beta
Calculated Molecular Weight 36 kDa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005218
Gene Symbol MAT2B
Gene ID (NCBI) 27430
RRIDAB_2882843
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAT2B antibody 67643-1-IgDownload protocol
IHC protocol for MAT2B antibody 67643-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols