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FMNL1 Monoclonal antibody

FMNL1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B12A4

Cat no : 67587-1-Ig

Synonyms

Formin-like protein 1, FMNL, CLL-associated antigen KW-13, 1B12A4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis using 67587-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis using 67587-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis of Jurkat using 67587-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NK-92 cells using FMNL1 Monoclonal antibody (67587-1-Ig)

    WB analysis of NK-92 using 67587-1-Ig

    NK-92 cells were subjected to SDS PAGE followed by western blot with 67587-1-Ig (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FMNL1 Monoclonal antibody (67587-1-Ig)

    IF Staining of U2OS using 67587-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (67587-1-Ig, Clone: 1B12A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inJurkat cells, NK-92 cells, HL-60 cells, K-562 cells, pig thymus tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67587-1-Ig targets FMNL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FMNL1 fusion protein Ag27322
Full Name formin-like 1
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC111564
Gene Symbol FMNL1
Gene ID (NCBI) 752
RRIDAB_2882796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 67587-1-Ig antibody detects the protein around 140-150 kDa which is similar to the results reported in publications.(PMID: 21868368, 22986496 , 23182705)

Protocols

Product Specific Protocols
WB protocol for FMNL1 antibody 67587-1-IgDownload protocol
IF protocol for FMNL1 antibody 67587-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols