HPD Monoclonal antibody

HPD Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67552-1-Ig
Clone No.3C9G2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (2)

Applications

WB, IHC, ELISA

4 HPPD, 4HPPD, GLOD3, HPD, HPPDase, PPD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, pig liver tissue, rat liver tissue, HepG2 cells, HEK-293 cells, HSC-T6 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67552-1-Ig targets HPD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, zebrafish
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HPD fusion protein Ag10928 Predict reactive species
Full Name 4-hydroxyphenylpyruvate dioxygenase
Calculated Molecular Weight 393 aa, 45 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC024287
Gene Symbol HPD
Gene ID (NCBI) 3242
RRIDAB_2882766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HPD antibody 67552-1-IgDownload protocol
IHC protocol for HPD antibody 67552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,zebrafishWB

Pest Manag Sci

In vivo fluorescent screening for HPPD-targeted herbicide discovery

Authors - Yi-Xuan Fu
humanIHC

Life Sci

Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest

Authors - Ruiwang Xie
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