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Septin 9 Monoclonal antibody

Septin 9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6F10

Cat no : 67548-1-Ig

Synonyms

AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, Septin 9, Septin D1, SINT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Septin 9 Monoclonal antibody (67548-1-Ig)

    WB analysis using 67548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Septin 9 Monoclonal antibody (67548-1-Ig)

    WB analysis using 67548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat colon tissue using Septin 9 Monoclonal antibody (67548-1-Ig)

    WB analysis of rat colon using 67548-1-Ig

    rat colon tissue were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig colon tissue, A549 cells, rat colon tissue, MCF-7 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67548-1-Ig targets Septin 9 in WB, ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 9 fusion protein Ag18047
Full Name septin 9
Calculated Molecular Weight 64 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Protocols

Product Specific Protocols
WB protocol for Septin 9 antibody 67548-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols