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  • Featured Product
  • KD/KO Validated

TMF1 Monoclonal antibody

TMF1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G12F2

Cat no : 67505-1-Ig

Synonyms

TMF, TATA element modulatory factor, ARA160, Androgen receptor-associated protein of 160 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TMF1 Monoclonal antibody (67505-1-Ig)

    WB analysis using 67505-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67505-1-Ig (TMF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TMF1 Monoclonal antibody (67505-1-Ig)

    WB analysis of HeLa using 67505-1-Ig

    WB result of TMF1 antibody (67505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMF1 transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TMF1 Monoclonal antibody (67505-1-Ig)

    IF Staining of HepG2 using 67505-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67505-1-Ig targets TMF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMF1 fusion protein Ag17666
Full Name TATA element modulatory factor 1
Calculated Molecular Weight 1093 aa, 123 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC126123
Gene Symbol TMF1
Gene ID (NCBI) 7110
RRIDAB_2882728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for TMF1 antibody 67505-1-IgDownload protocol
IF protocol for TMF1 antibody 67505-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Boyan (Verified Customer) (08-03-2022)

Very good signal in WB; a bit non specific staining in IF

  • Applications: Western Blot, Immunofluorescence