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HSD17B6 Monoclonal antibody

HSD17B6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D4G6

Cat no : 67496-1-Ig

Synonyms

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B6 Monoclonal antibody (67496-1-Ig)

    WB analysis using 67496-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67496-1-Ig (HSD17B6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using HSD17B6 Monoclonal antibody (67496-1-Ig)

    WB analysis of Daudi using 67496-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67496-1-Ig (HSD17B6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B6 Monoclonal antibody (67496-1-Ig)

    IHC staining of human liver cancer using 67496-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67496-1-Ig (HSD17B6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B6 Monoclonal antibody (67496-1-Ig)

    IHC staining of human liver cancer using 67496-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67496-1-Ig (HSD17B6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJAR cells, Daudi cells, HuH-7 cells, HepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67496-1-Ig targets HSD17B6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSD17B6 fusion protein Ag17330
Full Name hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
Calculated Molecular Weight 317 aa, 36 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC020710
Gene Symbol HSD17B6
Gene ID (NCBI) 8630
RRIDAB_2882720
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HSD17B6 antibody 67496-1-IgDownload protocol
IHC protocol for HSD17B6 antibody 67496-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols