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TRIM5 Monoclonal antibody

TRIM5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1G9

Cat no : 67492-1-Ig

Synonyms

RING finger protein 88, RNF88, TRIM5, TRIM5alpha, tripartite motif containing 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM5 Monoclonal antibody (67492-1-Ig)

    WB analysis using 67492-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67492-1-Ig (TRIM5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67492-1-Ig targets TRIM5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TRIM5 fusion protein Ag30059
Full Name tripartite motif-containing 5
Calculated Molecular Weight 493 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC021258
Gene Symbol TRIM5
Gene ID (NCBI) 85363
RRIDAB_2882717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM5 belongs to the large tripartite motif protein family, members of which typically are composed of 3 zinc-binding domains, a RING, unique B-box type 1 and B-box type 2 domains, followed by a coiled-coil (CC) region. TRIM proteins use homomultimerization to identify specific cell compartments. TRIM5 promotes innate immune signaling and that this activity is amplified by retroviral infection and interaction with the capsid lattice. TRIM5 has 6 variants termed alpha, beta, gamma, delta, epsilon, and iota with the MW of 56 kDa, 46 kDa, 40 kDa, 37 kDa, 31 kDa and 29 kDa. TRIM5 can form homodimers (~70 kDa) and homotrimers (90-160 kDa).

Protocols

Product Specific Protocols
WB protocol for TRIM5 antibody 67492-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols