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PSMG3 Monoclonal antibody

PSMG3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9C3

Cat no : 67466-1-Ig

Synonyms

C7orf48, hPAC3, PAC 3, PAC3, PSMG3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of A549 cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis of A549 using 67466-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis of K-562 using 67466-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis of HL-60 using 67466-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMG3 Monoclonal antibody (67466-1-Ig)

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMG3 Monoclonal antibody (67466-1-Ig)

    IF Staining of HeLa using 67466-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMG3 antibody (67466-1-Ig, Clone: 2H9C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, K-562 cells, HL-60 cells, A549 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67466-1-Ig targets PSMG3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PSMG3 fusion protein Ag8384
Full Name proteasome (prosome, macropain) assembly chaperone 3
Calculated Molecular Weight 13 kDa
Observed Molecular Weight13 kDa
GenBank Accession NumberBC004308
Gene Symbol PSMG3
Gene ID (NCBI) 84262
RRIDAB_2882695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSMG3 antibody 67466-1-IgDownload protocol
IF protocol for PSMG3 antibody 67466-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols