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MAT1A Monoclonal antibody

MAT1A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F1C1

Cat no : 67408-1-Ig

Synonyms

AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAT1A Monoclonal antibody (67408-1-Ig)

    WB analysis using 67408-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67408-1-Ig (MAT1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAT1A Monoclonal antibody (67408-1-Ig)

    IF Staining of HeLa using 67408-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAT1A antibody (67408-1-Ig, Clone: 2F1C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67408-1-Ig targets MAT1A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MAT1A fusion protein Ag3076
Full Name methionine adenosyltransferase I, alpha
Calculated Molecular Weight 395 aa, 44 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC018359
Gene Symbol MAT1A
Gene ID (NCBI) 4143
RRIDAB_2882649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A.

Protocols

Product Specific Protocols
WB protocol for MAT1A antibody 67408-1-IgDownload protocol
IF protocol for MAT1A antibody 67408-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols