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PTPN2 Monoclonal antibody

PTPN2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1D1

Cat no : 67388-1-Ig

Synonyms

PTPN2, PTPT, TC PTP, TCELLPTP, TCPTP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN2 Monoclonal antibody (67388-1-Ig)

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTPN2 Monoclonal antibody (67388-1-Ig)

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using PTPN2 Monoclonal antibody (67388-1-Ig)

    WB analysis of 4T1 using 67388-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPN2 Monoclonal antibody (67388-1-Ig)

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPN2 Monoclonal antibody (67388-1-Ig)

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, 4T1 cells, LNCaP cells, HEK-293T cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67388-1-Ig targets PTPN2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTPN2 fusion protein Ag29749
Full Name protein tyrosine phosphatase, non-receptor type 2
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC016727
Gene Symbol PTPN2
Gene ID (NCBI) 5771
RRIDAB_2882632
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

Protocols

Product Specific Protocols
WB protocol for PTPN2 antibody 67388-1-IgDownload protocol
IHC protocol for PTPN2 antibody 67388-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols