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TUSC3 Monoclonal antibody

TUSC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D3G12

Cat no : 67382-1-Ig

Synonyms

D8S1992, M33, MRT7, N33, OST3A, Protein N33, tumor suppressor candidate 3, TUSC3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TUSC3 Monoclonal antibody (67382-1-Ig)

    WB analysis of HeLa using 67382-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67382-1-Ig (TUSC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TUSC3 Monoclonal antibody (67382-1-Ig)

    WB analysis of HeLa using 67382-1-Ig

    WB result of TUSC3 antibody (67382-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC3 transfected hela cells.

  • Western Blot (WB) analysis of human placenta tissue using TUSC3 Monoclonal antibody (67382-1-Ig)

    WB analysis of human placenta using 67382-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67382-1-Ig (TUSC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67382-1-Ig targets TUSC3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TUSC3 fusion protein Ag9197
Full Name tumor suppressor candidate 3
Calculated Molecular Weight 347 aa, 40 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC010370
Gene Symbol TUSC3
Gene ID (NCBI) 7991
RRIDAB_2882629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUSC3 (tumor suppressor candidate 3), originally named N33, is a potential tumor supressor gene. Decreased expression of TUSC3 has been found in various cancers, including prostate cancer, pancreas cancer and ovary cancer. TUSC3 also known as OST3A, is identified as a part of the oligosaccharyl-transferase (OST) complex and plays a crucial role in protein N-glycosylation. TUSC3 mutations have been found in families with non-syndromic autosomal recessive mental retardation.

Protocols

Product Specific Protocols
WB protocol for TUSC3 antibody 67382-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols