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  • Featured Product
  • KD/KO Validated

UGDH Monoclonal antibody

UGDH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, pig and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E2E1

Cat no : 67360-1-Ig

Synonyms

GDH, UDP Glc dehydrogenase, UDP GlcDH, UDP glucose 6 dehydrogenase, UDP glucose dehydrogenase, UDPGDH, UGD, UGDH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UGDH Monoclonal antibody (67360-1-Ig)

    WB analysis using 67360-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using UGDH Monoclonal antibody (67360-1-Ig)

    WB analysis of rat brain using 67360-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UGDH Monoclonal antibody (67360-1-Ig)

    IHC staining of human liver cancer using 67360-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UGDH Monoclonal antibody (67360-1-Ig)

    IHC staining of human liver cancer using 67360-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UGDH Monoclonal antibody (67360-1-Ig)

    IF Staining of HepG2 using 67360-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGDH antibody (67360-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA431 cells, rat brain tissue, HeLa cells, HepG2 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67360-1-Ig targets UGDH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig samples.

Tested Reactivity Human, Mouse, Rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UGDH fusion protein Ag29747
Full Name UDP-glucose dehydrogenase
Calculated Molecular Weight 494 aa, 55 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC022781
Gene Symbol UGDH
Gene ID (NCBI) 7358
RRIDAB_2882612
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

Protocols

Product Specific Protocols
WB protocol for UGDH antibody 67360-1-IgDownload protocol
IHC protocol for UGDH antibody 67360-1-IgDownload protocol
IF protocol for UGDH antibody 67360-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Biochem Biophys Res Commun

UDP-glucose 6-dehydrogenase lessens sorafenib sensitivity via modulating unfolded protein response.

Authors - Bao Guo
  • KO Validated
View Article