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TDP-43 (C-terminal) Monoclonal antibody
TDP-43 (C-terminal) Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1G5F6
Cat no : 67345-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Y79 cells, SH-SY5Y cells, Neuro-2a cells, PC-12 cells |
Positive IHC detected in | mouse brain tissue, human gliomas tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:20000 |
Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IF | See 6 publications below |
Product Information
67345-1-Ig targets TDP-43 (C-terminal) in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | TAR DNA binding protein |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 43-45 kDa |
GenBank Accession Number | NM_007375 |
Gene Symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
RRID | AB_2882603 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TDP-43 (C-terminal) antibody 67345-1-Ig | Download protocol |
IHC protocol for TDP-43 (C-terminal) antibody 67345-1-Ig | Download protocol |
IF protocol for TDP-43 (C-terminal) antibody 67345-1-Ig | Download protocol |
FC protocol for TDP-43 (C-terminal) antibody 67345-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomedicines TDP-43 Pathology and Prionic Behavior in Human Cellular Models of Alzheimer's Disease Patients. | ||
ASN Neuro Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia | ||
Front Mol Neurosci TTBK1 and CK1 inhibitors restore TDP-43 pathology and avoid disease propagation in lymphoblast from Alzheimer's disease patients | ||
Neurobiol Dis CHCHD10S59L/+ mouse model: Behavioral and neuropathological features of frontotemporal dementia | ||
Neurobiol Dis Casein kinase 1 inhibitor avoids TDP-43 pathology propagation in a patient-derived cellular model of amyotrophic lateral sclerosis |