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HSP20 Monoclonal antibody, PBS Only (Capture)

HSP20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G7D2

Cat no : 67327-1-PBS

Synonyms

HspB6, Heat shock protein family B member 6, Heat shock protein beta-6, Heat shock protein beta 6, Heat shock 20 kDa-like protein p20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP20 Monoclonal antibody (67327-1-Ig)

    WB analysis using 67327-1-Ig (same clone as 67327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67327-1-Ig (HSP20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using HSP20 Monoclonal antibody (67327-1-Ig)

    IHC staining of human heart using 67327-1-Ig (same clone as 67327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67327-1-Ig (HSP20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using HSP20 Monoclonal antibody (67327-1-Ig)

    IF Staining of mouse heart using 67327-1-Ig (same clone as 67327-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-118 MG cells using HSP20 Monoclonal antibody (67327-1-Ig)

    IF Staining of U-118 MG using 67327-1-Ig (same clone as 67327-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-118 MG cells using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67327-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50552-2

    Cytometric bead array standard curve of MP50552-2

    Cytometric bead array standard curve of MP50552-2, HSPB6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67327-1-PBS. Detection antibody: 67327-4-PBS. Standard:Ag10484. Range: 0.098-100 ng/mL.

Product Information

67327-1-PBS targets HSP20 as part of a matched antibody pair:

MP50552-2: 67327-1-PBS capture and 67327-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HSP20 fusion protein Ag10484
Full Name heat shock protein, alpha-crystallin-related, B6
Calculated Molecular Weight 160 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC068046
Gene Symbol HSP20
Gene ID (NCBI) 126393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.