GNMT Monoclonal antibody

GNMT Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B5E3

Cat no : 67294-1-Ig

Synonyms

glycine N methyltransferase, GNMT



Tested Applications

Positive WB detected inrat liver tissue, pig liver tissue, mouse liver tissue, rat pancreas tissue, mouse pancreas tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67294-1-Ig targets GNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, Rat samples.

Tested Reactivity Human, Mouse, Pig, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNMT fusion protein Ag4598
Full Name glycine N-methyltransferase
Calculated Molecular Weight 295 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC032627
Gene Symbol GNMT
Gene ID (NCBI) 27232
RRIDAB_2882559
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

Protocols

Product Specific Protocols
WB protocol for GNMT antibody 67294-1-IgDownload protocol
IF protocol for GNMT antibody 67294-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.

Authors - Changhong Yang
humanWB

Cell Biochem Biophys

Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway

Authors - Long Miao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hua (Verified Customer) (11-11-2022)

Great antibody for WB. Strong signal and clean background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: Mouse liver
GNMT Antibody Western Blot validation (1:8000 dilution) in Mouse liver (Cat no:67294-1-Ig)