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PSG1 Monoclonal antibody

PSG1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C4

Cat no : 67269-1-Ig

Synonyms

Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F, B1G1

Validation Data Gallery

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  • Western Blot (WB) analysis of T-47D cells using PSG1 Monoclonal antibody (67269-1-Ig)

    WB analysis of T-47D using 67269-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67269-1-Ig (PSG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Monoclonal antibody (67269-1-Ig)

    IHC staining of human placenta using 67269-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Monoclonal antibody (67269-1-Ig)

    IHC staining of human placenta using 67269-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using PSG1 Monoclonal antibody (67269-1-Ig)

    IF Staining of human placenta using 67269-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using PSG1 Monoclonal antibody (67269-1-Ig)

    IF Staining of human placenta using 67269-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inT-47D cells, L02 cells, NCCIT cells, JAR cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67269-1-Ig targets PSG1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSG1 fusion protein Ag28604
Full Name pregnancy specific beta-1-glycoprotein 1
Calculated Molecular Weight 426 aa, 48 kDa
Observed Molecular Weight 54-70 kDa
GenBank Accession NumberBC022338
Gene Symbol PSG1
Gene ID (NCBI) 5669
RRIDAB_2882539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSG1 antibody 67269-1-IgDownload protocol
IHC protocol for PSG1 antibody 67269-1-IgDownload protocol
IF protocol for PSG1 antibody 67269-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols