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RHOQ/TC10 Monoclonal antibody

RHOQ/TC10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B9C7

Cat no : 67213-1-Ig

Synonyms

ARHQ, Ras like protein TC10, RASL7A, RHOQ, RHOQ/TC10, TC10, TC10A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    WB analysis using 67213-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of mouse liver using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of mouse liver using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of human colon cancer using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of human colon cancer using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue, pig brain tissue, fetal human brain tissue, Jurkat cells, A549 cells
Positive IHC detected inmouse liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67213-1-Ig targets RHOQ/TC10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RHOQ/TC10 fusion protein Ag12078
Full Name ras homolog gene family, member Q
Calculated Molecular Weight 205 aa, 23 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC070485
Gene Symbol RHOQ/TC10
Gene ID (NCBI) 23433
RRIDAB_2882505
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RHOQ/TC10 antibody 67213-1-IgDownload protocol
IHC protocol for RHOQ/TC10 antibody 67213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols