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FGFR1OP Monoclonal antibody

FGFR1OP Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F1D8

Cat no : 67207-1-Ig

Synonyms

FOP, FGFR1 oncogene partner, CEP43, Centrosomal protein 43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGFR1OP Monoclonal antibody (67207-1-Ig)

    WB analysis using 67207-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67207-1-Ig (FGFR1OP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FGFR1OP Monoclonal antibody (67207-1-Ig)

    WB analysis using 67207-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67207-1-Ig (FGFR1OP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of BT-549 cells using FGFR1OP Monoclonal antibody (67207-1-Ig)

    IF Staining of BT-549 using 67207-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed BT-549 cells using FGFR1OP antibody (67207-1-Ig, Clone: 1F1D8 ) at dilution of 1:2400 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cels, K-562 cells, U2OS cells
Positive IF/ICC detected inBT-549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1200-1:4800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67207-1-Ig targets FGFR1OP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FGFR1OP fusion protein Ag28671
Full Name FGFR1 oncogene partner
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC011902
Gene Symbol FGFR1OP
Gene ID (NCBI) 11116
RRIDAB_2882500
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGFR1OP, also named as FOP, is a centrosomal protein that is involved in the anchoring of microtubules to subcellular structures. Three isoforms have been produced by alternative splicing. A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma and progresses to acute myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for FGFR1OP antibody 67207-1-IgDownload protocol
IF protocol for FGFR1OP antibody 67207-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols