• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SQLE Monoclonal antibody, PBS Only (Capture)

SQLE Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9A2

Cat no : 67206-1-PBS

Synonyms

Squalene monooxygenase, squalene epoxidase, ERG1, EC:1.14.14.17, 1C9A2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using SQLE Monoclonal antibody (67206-1-Ig)

    WB analysis of A549 using 67206-1-Ig (same clone as 67206-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SQLE Monoclonal antibody (67206-1-Ig)

    WB analysis of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SQLE Monoclonal antibody (67206-1-Ig)

    WB analysis of HepG2 using 67206-1-Ig (same clone as 67206-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using SQLE Monoclonal antibody (67206-1-Ig)

    WB analysis of HSC-T6 using 67206-1-Ig (same clone as 67206-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using SQLE Monoclonal antibody (67206-1-Ig)

    WB analysis of L02 using 67206-1-Ig (same clone as 67206-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SQLE Monoclonal antibody (67206-1-Ig)

    IF Staining of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SQLE antibody (67206-1-Ig, Clone: 1C9A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50872-2

    Cytometric bead array standard curve of MP50872-2

    Cytometric bead array standard curve of MP50872-2, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-3-PBS. Standard:Ag3266. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50872-3

    Cytometric bead array standard curve of MP50872-3

    Cytometric bead array standard curve of MP50872-3, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-4-PBS. Standard:Ag3266. Range: 0.195-100 ng/mL.

  • Cytometric bead array standard curve of MP50872-4

    Cytometric bead array standard curve of MP50872-4

    Cytometric bead array standard curve of MP50872-4, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-5-PBS. Standard:null. Range: null.

Product Information

67206-1-PBS targets SQLE as part of a matched antibody pair:

MP50872-2: 67206-1-PBS capture and 67206-3-PBS detection (validated in Cytometric bead array)

MP50872-3: 67206-1-PBS capture and 67206-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SQLE fusion protein Ag3266
Full Name squalene epoxidase
Calculated Molecular Weight 574 aa, 64 kDa
Observed Molecular Weight 50-64 kDa
GenBank Accession NumberBC017033
Gene Symbol SQLE
Gene ID (NCBI) 6713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)