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PEPD Monoclonal antibody

PEPD Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H2A1

Cat no : 67202-1-Ig

Synonyms

Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis using 67202-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis of NCCIT using 67202-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis of HEK-293 using 67202-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis of human placenta using 67202-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PEPD Monoclonal antibody (67202-1-Ig)

    IHC staining of human breast cancer using 67202-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PEPD Monoclonal antibody (67202-1-Ig)

    IHC staining of human breast cancer using 67202-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, human placenta tissue, NCCIT cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67202-1-Ig targets PEPD in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEPD fusion protein Ag28713
Full Name peptidase D
Calculated Molecular Weight 493 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC015027
Gene Symbol PEPD
Gene ID (NCBI) 5184
RRIDAB_2882495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

Protocols

Product Specific Protocols
WB protocol for PEPD antibody 67202-1-IgDownload protocol
IHC protocol for PEPD antibody 67202-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Bioeng Biotechnol

Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.

Authors - Jie Chen
View Article