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  • Featured Product
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HSF1 Monoclonal antibody

HSF1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F10A5

Cat no : 67189-1-Ig

Synonyms

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSF1 Monoclonal antibody (67189-1-Ig)

    WB analysis using 67189-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67189-1-Ig (HSF1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HeLa cells using HSF1 Monoclonal antibody (67189-1-Ig)

    WB analysis of HeLa using 67189-1-Ig

    WB result of HSF1 antibody (67189-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human breast cancer using 67189-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human breast cancer using 67189-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human ovary tumor using 67189-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human ovary tumor using 67189-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67189-1-Ig targets HSF1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSF1 fusion protein Ag29061
Full Name heat shock transcription factor 1
Calculated Molecular Weight 529 aa, 57 kDa
Observed Molecular Weight 68-80 kDa
GenBank Accession NumberBC014638
Gene Symbol HSF1
Gene ID (NCBI) 3297
RRIDAB_2882484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

Protocols

Product Specific Protocols
WB protocol for HSF1 antibody 67189-1-IgDownload protocol
IHC protocol for HSF1 antibody 67189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Pancreas

Heat Shock Factor 1 in Relation to Tumor Angiogenesis and Disease Progression in Patients With Pancreatic Cancer.

Authors - Qiuman Lin
View Article