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GNA13 Monoclonal antibody
GNA13 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig and More (1)
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2G8F10
Cat no : 67188-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, Y79 cells, HepG2 cells, PC-12 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
67188-1-Ig targets GNA13 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GNA13 fusion protein Ag7316 |
| Full Name | guanine nucleotide binding protein (G protein), alpha 13 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC036756 |
| Gene Symbol | GNA13 |
| Gene ID (NCBI) | 10672 |
| RRID | AB_2882483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNA13 antibody 67188-1-Ig | Download protocol |
| IHC protocol for GNA13 antibody 67188-1-Ig | Download protocol |
| IF protocol for GNA13 antibody 67188-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis | ||
J Proteome Res Proteomic Investigation of Differential Interactomes of Glypican 1 and a Putative Disease-Modifying Variant of Ataxia | ||
J Clin Invest Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (12-29-2023) | Worked well for WB at 1:1000 in Glioma cells
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