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DMAP1 Monoclonal antibody

DMAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4E8

Cat no : 67183-1-Ig

Synonyms

DKFZp686L09142, DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425, SWC4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMAP1 Monoclonal antibody (67183-1-Ig)

    WB analysis using 67183-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67183-1-Ig (DMAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DMAP1 Monoclonal antibody (67183-1-Ig)

    IF Staining of HEK-293 using 67183-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DMAP1 antibody (67183-1-Ig, Clone: 1A4E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67183-1-Ig targets DMAP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DMAP1 fusion protein Ag28906
Full Name DNA methyltransferase 1 associated protein 1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC002855
Gene Symbol DMAP1
Gene ID (NCBI) 55929
RRIDAB_2882479
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).

Protocols

Product Specific Protocols
WB protocol for DMAP1 antibody 67183-1-IgDownload protocol
IF protocol for DMAP1 antibody 67183-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols