• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SHARPIN Monoclonal antibody

SHARPIN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A9E2

Cat no : 67177-1-Ig

Synonyms

DKFZp434N1923, hSIPL1, SHARPIN, SIPL1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SHARPIN Monoclonal antibody (67177-1-Ig)

    WB analysis using 67177-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67177-1-Ig (SHARPIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SHARPIN Monoclonal antibody (67177-1-Ig)

    WB analysis using 67177-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67177-1-Ig (SHARPIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA431 cells, HeLa cells, A549 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67177-1-Ig targets SHARPIN in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SHARPIN fusion protein Ag6367
Full Name SHANK-associated RH domain interactor
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC025244
Gene Symbol SHARPIN
Gene ID (NCBI) 81858
RRIDAB_2882473
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHARPIN antibody 67177-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols