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HSPA4 Monoclonal antibody

HSPA4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F2C12

Cat no : 67173-1-Ig

Synonyms

APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70, hsp70RY, HSPA4, RY

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis using 67173-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis of HEK-293 using 67173-1-Ig

    WB result of HSPA4 antibody (67173-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of Jurkat cells using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis of Jurkat using 67173-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis of pig brain using 67173-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, pig brain tissue, HepG2 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67173-1-Ig targets HSPA4 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen HSPA4 fusion protein Ag16624
Full Name heat shock 70kDa protein 4
Calculated Molecular Weight 840 aa, 94 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC110861
Gene Symbol HSPA4
Gene ID (NCBI) 3308
RRIDAB_2882469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors.

Protocols

Product Specific Protocols
WB protocol for HSPA4 antibody 67173-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols