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IRF8 Monoclonal antibody

IRF8 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A10A4

Cat no : 67142-1-Ig

Synonyms

H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IRF8 Monoclonal antibody (67142-1-Ig)

    WB analysis using 67142-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67142-1-Ig (IRF8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IRF8 Monoclonal antibody (67142-1-Ig)

    IF Staining of THP-1 using 67142-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRF8 antibody (67142-1-Ig, Clone: 3A10A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inRamos cells, Raji cells, Daudi cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67142-1-Ig targets IRF8 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IRF8 fusion protein Ag19909
Full Name ICSBP1
Calculated Molecular Weight 426 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC126247
Gene Symbol IRF8
Gene ID (NCBI) 3394
RRIDAB_2882441
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF8 also named as ICSBP1, is a transcription factor of the IFN regulatory factor (IRF) family. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)). It plays a negative regulatory role in cells of the immune system. It is predominantly in lymphoid tissues.

Protocols

Product Specific Protocols
WB protocol for IRF8 antibody 67142-1-IgDownload protocol
IF protocol for IRF8 antibody 67142-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols