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PTK2B Monoclonal antibody

PTK2B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A10G5

Cat no : 67141-1-Ig

Synonyms

CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTK2B Monoclonal antibody (67141-1-Ig)

    WB analysis using 67141-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PTK2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PTK2B Monoclonal antibody (67141-1-Ig)

    WB analysis of mouse brain using 67141-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PTK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue, K-562 cells, Ramos cells, pig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67141-1-Ig targets PTK2B in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTK2B fusion protein Ag11487
Full Name PTK2B protein tyrosine kinase 2 beta
Calculated Molecular Weight 1009 aa, 116 kDa
Observed Molecular Weight 112-115 kDa
GenBank Accession NumberBC042599
Gene Symbol PTK2B
Gene ID (NCBI) 2185
RRIDAB_2882440
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.

Protocols

Product Specific Protocols
WB protocol for PTK2B antibody 67141-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Discovery of electrophilic degraders that exploit SNAr chemistry

Authors - Zhe Zhuang
View Article