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RAB3D Monoclonal antibody

RAB3D Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C2F10

Cat no : 67118-1-Ig

Synonyms

D2 2, GOV, RAB16, RAB3D, RAD3D, Ras related protein Rab 3D

Validation Data Gallery

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  • Western Blot (WB) analysis of NCI-H1299 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of NCI-H1299 using 67118-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of A549 using 67118-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of HT-29 using 67118-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of THP-1 using 67118-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB3D Monoclonal antibody (67118-1-Ig)

    IHC staining of human colon cancer using 67118-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB3D Monoclonal antibody (67118-1-Ig)

    IHC staining of human colon cancer using 67118-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    IF Staining of A549 using 67118-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAB3D antibody (67118-1-Ig, Clone: 3C2F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNCI-H1299 cells, A549 cells, HT-29 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67118-1-Ig targets RAB3D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAB3D fusion protein Ag16784
Full Name RAB3D, member RAS oncogene family
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC016471
Gene Symbol RAB3D
Gene ID (NCBI) 9545
RRIDAB_2882421
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB3D antibody 67118-1-IgDownload protocol
IHC protocol for RAB3D antibody 67118-1-IgDownload protocol
IF protocol for RAB3D antibody 67118-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Tom (Verified Customer) (11-13-2020)

HEK293T cell extracts (10ug/lane). Primary antibody (1:2500) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-mouse HRP secondary antibody (1:10,000) incubated for 1 hour at RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: HEK293T
RAB3D Antibody Western Blot validation (1:2500 dilution) in HEK293T (Cat no:67118-1-Ig)