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PKC Delta Monoclonal antibody

PKC Delta Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B3E3

Cat no : 67113-1-Ig

Synonyms

MAY1, PKCD, PRKCD, Protein kinase C delta type, protein kinase C, delta

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of HeLa using 67113-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of MCF-7 using 67113-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of Jurkat using 67113-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of HSC-T6 using 67113-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of NIH/3T3 using 67113-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of HepG2 using 67113-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PKC Delta Monoclonal antibody (67113-1-Ig)

    WB analysis of A431 using 67113-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67113-1-Ig (PKC delta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, A431 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67113-1-Ig targets PKC Delta in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PKC Delta fusion protein Ag5631
Full Name protein kinase C, delta
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 78-80 kDa
GenBank Accession NumberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
RRIDAB_2882417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PKC Delta antibody 67113-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Differ

The PM20D1-NADA pathway protects against Parkinson's disease

Authors - Yunying Yang
View Article