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SAP102 Monoclonal antibody

SAP102 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A10

Cat no : 67102-1-Ig

Synonyms

Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using SAP102 Monoclonal antibody (67102-1-Ig)

    WB analysis of pig brain using 67102-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using SAP102 Monoclonal antibody (67102-1-Ig)

    WB analysis of mouse cerebellum using 67102-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SAP102 Monoclonal antibody (67102-1-Ig)

    WB analysis of rat brain using 67102-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SAP102 Monoclonal antibody (67102-1-Ig)

    IHC staining of mouse brain using 67102-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67102-1-Ig (SAP102 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SAP102 Monoclonal antibody (67102-1-Ig)

    IHC staining of mouse brain using 67102-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67102-1-Ig (SAP102 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inpig brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67102-1-Ig targets SAP102 in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SAP102 fusion protein Ag28589
Full Name discs, large homolog 3 (Drosophila)
Calculated Molecular Weight 817 aa, 90 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC093864
Gene Symbol SAP102
Gene ID (NCBI) 1741
RRIDAB_2882407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synapse-associated protein 102 (SAP102), also known as DLG3, is a MAGUK that is highly expressed early in development and mediates receptor trafficking during synaptogenesis. The importance of SAP102 function in brain development is demonstrated by the fact that SAP102 mutations have been identified in human patients with nonsyndromic X-linked mental retardation (XLMR).SAP102 has critical roles in early cortical synapse development.

Protocols

Product Specific Protocols
WB protocol for SAP102 antibody 67102-1-IgDownload protocol
IHC protocol for SAP102 antibody 67102-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols