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ESD Monoclonal antibody

ESD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F5E1

Cat no : 67069-2-Ig

Synonyms

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ESD Monoclonal antibody (67069-2-Ig)

    WB analysis using 67069-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-2-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHCT 116 cells, Caco-2 cells, HepG2 cells, Jurkat cells, K-562 cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67069-2-Ig targets ESD in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESD fusion protein Ag7487
Full Name esterase D/formylglutathione hydrolase
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC001169
Gene Symbol ESD
Gene ID (NCBI) 2098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

Protocols

Product Specific Protocols
WB protocol for ESD antibody 67069-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols