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  • Featured Product
  • KD/KO Validated

LSM12 Monoclonal antibody

LSM12 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2E3G3

Cat no : 67029-1-Ig

Synonyms

Protein LSM12, 2E3G3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LSM12 Monoclonal antibody (67029-1-Ig)

    WB analysis using 67029-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67029-1-Ig (LSM12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LSM12 Monoclonal antibody (67029-1-Ig)

    IHC staining of human colon cancer using 67029-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using LSM12 Monoclonal antibody (67029-1-Ig)

    FC experiment of HeLa using 67029-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug LSM12 Monoclonal antibody (67029-1-Ig, Clone:2E3G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67029-1-Ig targets LSM12 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LSM12 fusion protein Ag28782
Full Name LSM12 homolog (S. cerevisiae)
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC044587
Gene Symbol LSM12
Gene ID (NCBI) 124801
RRIDAB_2882344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LSM12 antibody 67029-1-IgDownload protocol
IHC protocol for LSM12 antibody 67029-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Signal

Convergent activation of Ca2+ permeability in two-pore channel 2 through distinct molecular routes

Authors - Ryo Saito
  • KO Validated
View Article