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NFS1 Monoclonal antibody

NFS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A10A7

Cat no : 67021-1-Ig

Synonyms

HUSSY 08, IscS, NFS1, NIFS

Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HSC-T6 using 67021-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HepG2 using 67021-1-Ig

    WB result of NFS1 antibody (67021-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells.

  • Western Blot (WB) analysis of NIH/3T3 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of NIH/3T3 using 67021-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of RAW 264.7 using 67021-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HeLa using 67021-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HEK-293 using 67021-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NFS1 Monoclonal antibody (67021-1-Ig)

    WB analysis of HepG2 using 67021-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHSC-T6 cells, HepG2 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67021-1-Ig targets NFS1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFS1 fusion protein Ag8017
Full Name NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC018471
Gene Symbol NFS1
Gene ID (NCBI) 9054
RRIDAB_2918480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for NFS1 antibody 67021-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols